PCNA 抗体 (CF®740)
Quick Overview for PCNA 抗体 (CF®740) (ABIN7659196)
抗原
See all PCNA 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- PCNA(PC10), CF740 conjugate
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产品特性
- Recognizes a non-histone protein of 36 kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Rat PCNA/Protein A fusion protein
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亚型
- IgG2a, kappa
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anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 151-261) antibody
PCNA 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261) antibodyPCNA 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal S1 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, IHC (fro), FISH 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibodyPCNA 适用: 人, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7H4F8 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 53-196) antibodyPCNA 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 7H4F8 unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 201-261) antibodyPCNA 适用: 人, 小鼠, 大鼠, 小鸡 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibodyPCNA 适用: 人, 小鼠, 大鼠, Pig, 犬, 兔, 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Proliferating Cell Nuclear Antigen (PCNA) antibodyPCNA 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.25-0.5 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Tonsil or reactive lymph node
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- PCNA (Proliferating Cell Nuclear Antigen (PCNA))
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别名
- PCNA
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背景
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Synonyms: Cyclin, DNA polymerase delta auxiliary protein, Mutagen-sensitive 209 protein, PCNAR, Polymerase delta accessory protein
Gene Symbol: PCNA
Tissue Expression: Proliferating cells
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分子量
- 36 kDa
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基因ID
- 5111
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UniProt
- P12004
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途径
- Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy
抗原
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